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Open Access Journal Article

Detection of 16 kDa Protein from Mycobacterium Tuberculosis Using Interdigitated Electrode

by S.S. Rani a  and  Subash C.B. Gopinath a,b,c,*
a
Institute of Nano Electronic Engineering, Malaysia
b
Faculty of Chemical Engineering Technology, Universiti Malaysia Perlis, 02600 Perlis, Malaysia
c
Centre of Excellence for Nanobiotechnology and Nanomedicine (CoExNano), Faculty of Applied Sciences, AIMST University, Semeling, 08100 Kedah, Malaysia
*
Author to whom correspondence should be addressed.
BAB  2022, 1; 1(1), 1; https://doi.org/10.58567/bab01010001
Received: 15 November 2022 / Accepted: 5 December 2022 / Published Online: 26 December 2022
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Abstract

 In the past, researchers investigated the immune response acting against mycobacterial antigens in tuberculosis (TB). Several ELISA tests have been conducted for the diagnosis of tuberculosis. Other researchers manipulated 16 kDa antigen from Mycobacterium Tuberculosis to develop a tuberculosis diagnostic test while some others studied the subspecies of M. tuberculosis. In the present study, we explored the possibilities of detecting 16 kDa protein derived from M. tuberculosis, on an interdigitated electrode (IDE). Measurements with Electrochemical impedance spectroscopy and current-volt systems were demonstrated using antibody as the probe.  Further, the involvement of gold nanoparticle for the signal-enhancement and high-performance are evidenced. The specificity in the serum containing samples is used to show the clinical relevancy. 16 kDa antigen used in this experiment is highly reliable for TB diagnosis as it largely expresses and can be implemented in other sensing system.

Keywords: Biomarker; Dielectrode sensor; Pathogen; Nanobiosensor;
Copyright: © 2022 by Rani and Gopinath. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) (Creative Commons Attribution 4.0 International License). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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ACS Style
Rani, S.; Gopinath, S. C. Detection of 16 kDa Protein from Mycobacterium Tuberculosis Using Interdigitated Electrode. Biomaterials and Biosensors, 2022, 1, 1. https://doi.org/10.58567/bab01010001
AMA Style
Rani S, Gopinath S C. Detection of 16 kDa Protein from Mycobacterium Tuberculosis Using Interdigitated Electrode. Biomaterials and Biosensors; 2022, 1(1):1. https://doi.org/10.58567/bab01010001
Chicago/Turabian Style
Rani, S.S.; Gopinath, Subash C. 2022. "Detection of 16 kDa Protein from Mycobacterium Tuberculosis Using Interdigitated Electrode" Biomaterials and Biosensors 1, no.1:1. https://doi.org/10.58567/bab01010001
APA style
Rani, S., & Gopinath, S. C. (2022). Detection of 16 kDa Protein from Mycobacterium Tuberculosis Using Interdigitated Electrode. Biomaterials and Biosensors, 1(1), 1. https://doi.org/10.58567/bab01010001

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